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human coronary artery endothelial cells hcaecs  (ATCC)


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    ATCC human coronary artery endothelial cells hcaecs
    Human Coronary Artery Endothelial Cells Hcaecs, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+coronary+artery+endothelial+cells+hcaecs/Primary+Coronary+Artery+Endothelial+Cells%3B+Normal%2C+Human/pm41421410-61-0-10
    Average 95 stars, based on 160 article reviews
    human coronary artery endothelial cells hcaecs - by Bioz Stars, 2026-09
    95/100 stars

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    Cell Culture:

    Article Title: Plasma proteomic profiling reveals that SERPINE1 is a potential biomarker associated with coronary artery lesions in Kawasaki disease.
    Article Snippet: The stained sections were photographed using a fluorescence microscope (Olympus, Tokyo, Japan). .. Human coronary artery endothelial cells (HCAECs) were sourced from ATCC and cultured at 37 ◦C with 5 % CO2 in DMEM (HyClone) supplemented with 10 % FBS (Gibco), 100 U/mL penicillin, and 100 mg/L streptomycin (Invitrogen). ..

    Article Title: NFIL3 aggravates human coronary artery endothelial cell injury by promoting ITGAM transcription in Kawasaki disease.
    Article Snippet: .. Human coronary artery endothelial cells (HCAECs) purchased from American Type Culture Collection (ATCC) were cultured (37°C, 5% CO2) in a medium appended with growth factors and 10% fetal bovine serum. ..

    Article Title: Long Non-Coding RNA PCAT19 Suppresses Cell Proliferation and Angiogenesis in Coronary Artery Disease through Interaction with GCNT2.
    Article Snippet: LncRNAs involvement in heart disease, however, the effect of lncRNA prostate cancer‐associated transcript 19 (PCAT19) in coronary artery disease (CAD) remains unclear.. In the current study, we aimed to verify the role of PCAT19 in CAD.. We first investigated the differentially expressed lncRNAs in different Genes Expression Omnibus (GEO) database.

    Article Title: Therapeutic potential of oral edaravone in diabetic endothelial dysfunction through NF-κB and p38 pathway inhibition.
    Article Snippet: Objective: Diabetes mellitus is a chronic metabolic disorder frequently associated with endothelial dysfunction, a major contributor to cardiovascular complications.. This study aimed to investigate the protective effects of oral edaravone (OED) on endothelial dysfunction in diabetic models and explore its underlying molecular

    Article Title: The Potential of Downregulated Serum miR-1282 to Predict Disease Onset and Short-Term Prognosis in Acute Coronary Syndrome Patients
    Article Snippet: .. Human coronary artery endothelial cells (HCAECs) were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM medium supplemented with 10% fetal bovine serum. .. Using Lipofectamine 3000 reagent (Invitrogen), the cells were transfected with miR-1282 mimic, miR-1282 inhibitor, and their corresponding negative controls; as well as with QKI overexpression plasmid (OE-QKI) and its negative control (OE-NC), all of which were provided by GenePharma (Shanghai, China).



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    MRMP mediates upregulation of MPC1. (A) MPC1 mRNA expression in MRMP-infected <t>HCAECs</t> was determined using qRT-PCR ( n = 3). (B,C) MPC1 protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.
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    Image Search Results


    MRMP mediates upregulation of MPC1. (A) MPC1 mRNA expression in MRMP-infected HCAECs was determined using qRT-PCR ( n = 3). (B,C) MPC1 protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: MRMP mediates upregulation of MPC1. (A) MPC1 mRNA expression in MRMP-infected HCAECs was determined using qRT-PCR ( n = 3). (B,C) MPC1 protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Expressing, Infection, Quantitative RT-PCR, Western Blot

    UK5099 alleviates MRMP-induced mitochondrial damage (A) mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey’s post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: UK5099 alleviates MRMP-induced mitochondrial damage (A) mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey’s post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Infection, Quantitative RT-PCR, Expressing, Western Blot

    UK5099 alleviates MRMP-induced pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay. (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: UK5099 alleviates MRMP-induced pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay. (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay, Expressing, Infection, Western Blot

    MG132-mediated PINK1 deficiency induces mitochondrial damage. (A) Mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E,F) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: MG132-mediated PINK1 deficiency induces mitochondrial damage. (A) Mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E,F) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Infection, Quantitative RT-PCR, Expressing, Western Blot

    MG132-mediated PINK1 deficiency induces pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay ( n = 3). (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: MG132-mediated PINK1 deficiency induces pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay ( n = 3). (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay, Expressing, Infection, Western Blot

    MPC1 induces pyroptosis of HCAECs via inhibiting PINK1-dependent mitophagy. (A–D) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). (E,F) Cytokine release was determined using ELISA ( n = 3). (G) Cell viability was determined using CCK-8 assay ( n = 3). (H) Cytotoxicity was determined using LDH assay ( n = 3). (I,J) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (K–N) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Journal: Frontiers in Pediatrics

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

    doi: 10.3389/fped.2026.1731155

    Figure Lengend Snippet: MPC1 induces pyroptosis of HCAECs via inhibiting PINK1-dependent mitophagy. (A–D) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). (E,F) Cytokine release was determined using ELISA ( n = 3). (G) Cell viability was determined using CCK-8 assay ( n = 3). (H) Cytotoxicity was determined using LDH assay ( n = 3). (I,J) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (K–N) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

    Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

    Techniques: Expressing, Infection, Western Blot, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay